Carmine Isacco was born on September 20, 1970, in Toronto, Ontario. He starred as a goalkeeper for the University of Maryland from 1989 to 1992. In 1992 he joined Toronto Italia in the National Soccer League.

He finished the 1993 Canadian National Soccer League regular season placed as second in the Goalkeepers Standings. Carmen played in 15 league games and let in 11 goals, having a goals against average of 0.74.

Carmen later played for Milwaukee Rampage, Toronto Shooting Stars, Milwaukee Wave, Rochester Rhinos, Edmonton Drillers, Toronto Lynx, Buffalo Blizzard and Toronto Supra.